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Pay Scale: STANDARD GRADE 6

Salary (£):  £32,332 to £38,205 per annum

Location: Department of Biochemistry, South Parks Road, Oxford

Contract type: Fixed Term Contract up to 6th June 2025

Hours: Full Time (37.5 hours per week)

 

THIS IS A RE-ADVERTISEMENT, PREVIOUS APPLICANTS NEED NOT APPLY

 

About the role

We are seeking to appoint a Research Technician and Laboratory Manager to join the lab of Prof Nick Lakin.  The research team investigates the molecular basis of DNA repair and how this contributes to maintenance of genome integrity. Our specific interests are in deciphering the role of poly-ADP-ribose polymerases (PARPs) in the detection and signalling of DNA damage and how this promotes DNA repair. We have significant expertise in studying these processes in human cells and model organisms.

 

The Research Technician will manage our eukaryotic cell culture library and other lab stocks in addition to providing experimental support to a variety of projects. Tasks will involve cell-based assays for DNA repair, using genome editing technologies to generate cell lines and recombinant DNA technology.  The Research Technician will also act as laboratory manager for the research group, proactively maintaining stocks, equipment and facilities, health and safety protocols, and creating an effective working environment.

 

This full-time fixed-term post is funded by the Medical Research Council up to 6th June 2025 in the first instance. The actual starting salary offered will be based on qualifications and relevant skills acquired and will also be determined by the funding available.

 

About you

You will hold a BSc Degree in an area of molecular and cell biology, genetics or biochemistry. You should have experience of mammalian cell culture, including clonal cell line generation and recombinant protein expression using mammalian expression systems. You will also have experience of employing cell-based assays to assess mechanisms of cell function. Experience in manipulating gene expression by siRNA and/or genome editing is also desirable.  You will have experience of working in laboratory environment and be able to work effectively with other lab members as part of a team.  Good organisational and management skills are expected in order to manage facilities and equipment effectively, together with being proactive and capable of taking the lead in maintaining an effective and orderly lab environment.

 

Application Process

You will be required to upload a supporting statement, setting out how you meet the selection criteria, curriculum vitae and the names and contact details of two referees as part of your online application.

 

Only applications received before 12 noon on Friday 6th October 2023 can be considered.  Interviews for shortlisted candidates will be held as soon as possible thereafter.

 

Committed to equality and valuing diversity
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"""_________________________________________________________________________ DEPARTMENT OF BIOCHEMISTRY Job Description Job title Research Technician and Laboratory Manager Division Medical Sciences Division Department Department of Biochemistry Location Dorothy Crowfoot Hodgkin Building, South Parks Road, Oxford OX1 3QU Grade and salary Grade 6: £32,332 - £38,205 per annum Hours Full-time (37.5 hours per week) Contract type Fixed Term Contract up to 6th June 2025 Reporting to Professor Nick Lakin Vacancy reference 168026 Additional information You are required to submit a CV and a supporting statement with your application, outlining how you meet each of the selection criteria for the role (see below for details). Your application will not be processed if you do not include both documents Research topic The role of PARPs in DNA repair and genome stability Principal Investigator / supervisor Professor Nick Lakin Project team Lakin Lab Project web site https://www.bioch.ox.ac.uk/research/lakin Funding partner Medical Research Council Recent publications Sharma A.B., Ramlee M.K., Kosmin J., Higgs M.R., Wolstenholme A., Ronson G.E, Jones D., Ebner D., Shamkhi N.F., Sims D., Wijnhoven P.W.G., Forment J., Gibbs-Seymour I. and Lakin N.D. (2023). C16orf72/HAPSTR1/TAPR1 functions with BRCA1/Senataxin to modulate replication-associated R-loops and confer resistance to PARP disruption. Nature Communications. 14:5003; doi: 10.1038/s41467-023-40779-9. Richards F., Llorca-Cardenosa M.J., Langton J., Buch-Larsen S.C., Shamki N.F., Sharma A.B., Nielsen M.L. and Lakin N.D. (2023). Regulation of Rad52-dependent replication fork recovery through serine ADPribosylation of PolD3. Nature Communications. 14:4310; doi: 10.1038/s41467-023-40071-w. Brustel J., Muramoto T., Fumimoto K., Ellins J., Pears C.J. and Lakin N.D. (2022). Linking DNA repair and cell cycle progression through serine ADP-ribosylation of histones. Nature Communications. 13:185. DOI: 10.1038/s41467-021-27867-4. Ronson G.E., Piberger A.L., Higgs M.R., Olsen A.L., Stewart G.S., McHugh P.J., Petermann E. and Lakin N.D. (2018). PARP1 and PARP2 stabilise replication forks at base excision repair intermediates through Fbh1-dependent Rad51 regulation. Nature Communications. 9:746. DOI: 10.1038/s41467-018-03159-2. Kolb A.-L., Gunn A.R. and Lakin N.D. (2017). Redundancy between nucleases required for homologous recombination promotes PARP inhibitor resistance in the eukaryotic model organism Dictyostelium. Nucleic Acids Research. 45:10056-10067. The role The Research Technician will join the research team of Professor Nick Lakin that investigates the molecular basis of DNA repair and how this contributes to maintenance of genome integrity. Our specific interests are in deciphering the role of poly-ADP-ribose polymerases (PARPs) in the detection and signalling of DNA damage and how this promotes DNA repair. We have significant expertise in studying these processes in human cells and model organisms. We are based at the Department of Biochemistry, University of Oxford and have access to a wide variety of technologies to facilitate these studies including cutting edge genome editing, microscopy, flow cytometry, proteomics, genome sequencing and high throughput cell screening facilities. The Research Technician will manage our eukaryotic cell culture library and other lab stocks in addition to providing experimental support to a variety of projects. Tasks will involve using genome editing technologies to generate cell lines, recombinant DNA technology and cell-based assays for DNA repair. The Research Technician will also act as laboratory manager for the research group, proactively maintaining stocks, equipment and facilities, health and safety protocols, and creating an effective working environment. Responsibilities/duties The Research Technician will perform research by generating and maintaining cell lines using genome editing to support a variety of projects in the laboratory and will also act as laboratory manager for the research team, maintaining stocks, equipment and facilities. This candidate will:  Manage own research and experimental activities, within guidelines provided by colleagues.  Maintain and generate cell lines using genome editing and other molecular and cellular assays.  Be responsible for eukaryotic cell culture room and maintenance of stock cells.  Select, follow, and adapt experimental protocols.  Use specialist scientific equipment in a laboratory environment.  Gather, analyse, and present scientific data from a variety of sources. 168026 Research Assistant Gr 6 JD - Lakin 2  Be responsible for general laboratory management and administration, including lab stock management, health and safety and control of laboratory consumables.  Be responsible for safety documentation, protocols and archives. Selection criteria Essential The successful candidate will: 1. Possess a Bachelor of Science degree in an area of molecular and cell biology, genetics or biochemistry. 2. Have experience of lab management and supporting a research team, including the organisational and management skills required to manage facilities and equipment efficiently. 3. Experience of mammalian cell culture, clonal cell line generation and maintenance, expression of recombinant proteins in mammalian cells. 4. Experience of cell-based assays to assess a variety of cellular functions. 5. Work effectively with other laboratory members as part of a team. Desirable 1. Experience of manipulating gene function such as siRNA knockdown and/or CRISPR-Cas9 genome editing. 2. Experience in managing laboratory facilities. 3. Experience of cloning and molecular biology. Pre-employment screening Standard checks If you are offered the post, the offer will be subject to standard pre-employment checks. You will be asked to provide: proof of your right-to-work in the UK; proof of your identity; and (if we haven’t done so already) we will contact the referees you have nominated. You will also be asked to complete a health declaration so that you can tell us about any health conditions or disabilities for which you may need us to make appropriate adjustments. Please read the candidate notes on the University’s pre-employment screening procedures at: https://www.jobs.ox.ac.uk/pre-employment-checks Hazard-specific / Safety-critical duties This job includes hazards or safety-critical activities. If you are offered the post, you will be asked to complete a health questionnaire which will be assessed by our Occupational Health Service, and the offer of employment will be subject a successful outcome of this assessment. The hazards or safety-critical duties involved are as follows:  Lone Working  Work in hot or cold environments  Driving on University business  Working with infectious pathogens (hazard group 2/3) - Hazard Group 3 pathogens 168026 Research Assistant Gr 6 JD - Lakin 3  Work with any substance which has any of the following pictograms on their MSDS: About the University of Oxford Welcome to the University of Oxford. We aim to lead the world in research and education for the benefit of society both in the UK and globally. Oxford’s researchers engage with academic, commercial and cultural partners across the world to stimulate high-quality research and enable innovation through a broad range of social, policy and economic impacts. We believe our strengths lie both in empowering individuals and teams to address fundamental questions of global significance, while providing all our staff with a welcoming and inclusive workplace that enables everyone to develop and do their best work. Recognising that diversity is our strength, vital for innovation and creativity, we aspire to build a truly diverse community which values and respects every individual’s unique contribution. While we have long traditions of scholarship, we are also forward-looking, creative and cutting-edge. Oxford is one of Europe's most entrepreneurial universities and we rank first in the UK for university spin-outs, and in recent years we have spun out 15-20 new companies every year. We are also recognised as leaders in support for social enterprise. Join us and you will find a unique, democratic and international community, a great range of staff benefits and access to a vibrant array of cultural activities in the beautiful city of Oxford. For more information, please visit www.ox.ac.uk/about/organisation. Department of Biochemistry The Department of Biochemistry in Oxford was established in 1920 and is now one of the largest in Europe. Situated in an attractive area close to the University Parks and River Cherwell, the Department is housed in the University Science Area and is currently undergoing a major expansion programme centred on the recently completed and award-winning New Biochemistry Building. The Science Area includes the Radcliffe Science Library and the Natural History Museum, and is conveniently located for easy access to the town centre and colleges. The department includes research laboratories working in the areas of Cell Biology, Development and Genetics; Chromosomal and RNA Biology; Infection and Disease Processes; Microbiology and Systems Biology; and Structural Biology and Molecular Biophysics. It is particularly well equipped with an extensive computer network, all the basic hardware essential in today's research, together with an excellent range of state-of-the-art specialist equipment. For more information please visit: http://www.bioch.ox.ac.uk/ The University of Oxford is a member of the Athena SWAN Charter and holds an institutional Bronze Athena SWAN award. The Department of Biochemistry is strongly committed to equality and valuing diversity and we operate a flexible working policy for all staff. The Department holds a departmental Silver Athena SWAN award to recognise advancement of gender equality: representation, progression and success for all. Medical Sciences Division The Medical Sciences Division is an internationally recognized centre of excellence for biomedical and clinical research and teaching, and the largest academic division in the University of Oxford. It includes 15 clinical departments and 5 non-clinical departments. 168026 Research Assistant Gr 6 JD - Lakin 4 World-leading programmes, housed in state-of-the-art facilities, cover the full range of scientific endeavour from the molecule to the population. With our NHS partners we also foster the highest possible standards in patient care. For more information please visit: http://www.ox.ac.uk/divisions/medical_sciences.html How to apply Applications are made through our online recruitment portal. Information about how to apply is available on our Jobs website https://www.jobs.ox.ac.uk/how-to-apply. Your application will be judged solely on the basis of how you demonstrate that you meet the selection criteria stated in the job description. As part of your application you will be asked to provide details of two referees and indicate whether we can contact them now. You will be asked to upload a CV and a supporting statement. The supporting statement must explain how you meet each of the selection criteria for the post using examples of your skills and experience. This may include experience gained in employment, education, or during career breaks (such as time out to care for dependants). Please upload all documents as PDF files with your name and the document type in the filename. All applications must be received by midday UK time on the closing date stated in the online advertisement. Information for priority candidates A priority candidate is a University employee who is seeking redeployment because they have been advised that they are at risk of redundancy, or on grounds of ill-health/disability. Priority candidates are issued with a redeployment letter by their employing department(s). If you are a priority candidate, please ensure that you attach your redeployment letter to your application (or email it to the contact address on the advert if the application form used for the vacancy does not allow attachments). If you need help Application FAQs, including technical troubleshooting https://staff.web.ox.ac.uk/recruitment-support-faqs advice is available at: Non-technical questions about this job should be addressed to the recruiting department directly, please email jobs@bioch.ox.ac.uk To return to the online application at any stage, please go to: www.recruit.ox.ac.uk. Please note that you will receive an automated email from our online recruitment portal to confirm receipt of your application. Please check your spam/junk mail if you do not receive this email. 168026 Research Assistant Gr 6 JD - Lakin 5 Important information for candidates Data Privacy Please note that any personal data submitted to the University as part of the job application process will be processed in accordance with the GDPR and related UK data protection legislation. For further information, please see the University’s Privacy Notice for Job Applicants at: https://compliance.admin.ox.ac.uk/job-applicant-privacy-policy. The University’s Policy on Data Protection is available at: https://compliance.admin.ox.ac.uk/data-protection-policy. The University’s policy on retirement The University operates an Employer Justified Retirement Age (EJRA) for very senior research posts at grade RSIV/D35 and clinical equivalents E62 and E82, which with effect from 1 October 2023 will be 30 September before the 70th birthday. The justification for this is explained at: https://hr.admin.ox.ac.uk/the-ejra. For existing employees on these grades, any employment beyond the retirement age is subject to approval through the procedures: https://hr.admin.ox.ac.uk/the-ejra. There is no normal or fixed age at which staff in posts at other grades have to retire. Staff at these grades may elect to retire in accordance with the rules of the applicable pension scheme, as may be amended from time to time. Equality of opportunity Entry into employment with the University and progression within employment will be determined only by personal merit and the application of criteria which are related to the duties of each particular post and the relevant salary structure. In all cases, ability to perform the job will be the primary consideration. No applicant or member of staff shall be discriminated against because of age, disability, gender reassignment, marriage or civil partnership, pregnancy or maternity, race, religion or belief, sex, or sexual orientation. The University’s Policy on Information Security Awareness The information security awareness training is compulsory for all University staff; and as part of our responsibility as a Department, ALL employees of the Department will be required to complete the online information security awareness module, which provides a combination of information, case studies and links to additional resources relating to information security. You will be expected to complete this course as part of your induction process, on your first day working in the Department of Biochemistry. This training will also need to be completed in order to successfully complete your probationary period. In order to ensure that we are compliant and up-to-date with the information security awareness training, we need to ensure that all staff members have completed the latest course, which may be accessed from the Information Security’s website by using the following link: https://www.infosec.ox.ac.uk/guidance-policy/training-and-awareness You will also be required to undertake this course on an annual basis, in order to satisfy the security awareness training requirements of the University’s Information Security Policy. 168026 Research Assistant Gr 6 JD - Lakin 6 Benefits of working at the University Employee benefits University employees enjoy 38 days’ paid holiday, generous pension schemes, travel discounts, and a variety of professional development opportunities. Our range of other employee benefits and discounts also includes free entry to the Botanic Gardens and University colleges, and discounts at University museums. See https://hr.admin.ox.ac.uk/staff-benefits University Club and sports facilities Membership of the University Club is free for all University staff. The University Club offers social, sporting, and hospitality facilities. Staff can also use the University Sports Centre on Iffley Road at discounted rates, including a fitness centre, powerlifting room, and swimming pool. See www.club.ox.ac.uk and https://www.sport.ox.ac.uk/. Information for staff new to Oxford If you are relocating to Oxfordshire from overseas or elsewhere in the UK, the University's Welcome Service website includes practical information about settling in the area, including advice on relocation, accommodation, and local schools. See https://welcome.ox.ac.uk/ There is also a visa loan scheme to cover the costs of UK visa applications for staff and their dependants. See https://staffimmigration.admin.ox.ac.uk/visa-loan-scheme Family-friendly benefits With one of the most generous family leave schemes in the Higher Education sector, and a range of flexible working options, Oxford aims to be a family-friendly employer. We also subscribe to the Work+Family Space, a service that provides practical advice and support for employees who have caring responsibilities. The service offers a free telephone advice line, and the ability to book emergency backup care for children, adult dependents and elderly relatives. See https://hr.admin.ox.ac.uk/my-familycare The University has excellent childcare services, including five University nurseries as well as Universitysupported places at many other private nurseries. For full details, including how to apply and the costs, see https://childcare.admin.ox.ac.uk/ Disabled staff We are committed to supporting members of staff with disabilities or long-term health conditions. For further details, including information about how to make contact, in confidence, with the University’s Staff Disability Advisor, see https://edu.admin.ox.ac.uk/disability-support Staff networks The University has a number of staff networks including the Oxford Research Staff Society, BME staff network, LGBT+ staff network and a disabled staff network. You can find more information at https://edu.admin.ox.ac.uk/networks The University of Oxford Newcomers' Club The University of Oxford Newcomers' Club is an organisation run by volunteers that aims to assist the partners of new staff settle into Oxford, and provides them with an opportunity to meet people and make connections in the local area. See www.newcomers.ox.ac.uk. 168026 Research Assistant Gr 6 JD - Lakin 7 """^^ . "way/100019761" . "New Biochemistry" . . "281" . """**Pay Scale:** STANDARD GRADE 6 **Salary ( £)**: £32,332 to £38,205 per annum **Location: **Department of Biochemistry, South Parks Road, Oxford **Contract type: **Fixed Term Contract up to 6th June 2025 **Hours:** Full Time (37.5 hours per week) **THIS IS A RE-ADVERTISEMENT, PREVIOUS APPLICANTS NEED NOT APPLY** **About the role** We are seeking to appoint a Research Technician and Laboratory Manager to join the lab of Prof Nick Lakin. The research team investigates the molecular basis of DNA repair and how this contributes to maintenance of genome integrity. Our specific interests are in deciphering the role of poly-ADP-ribose polymerases (PARPs) in the detection and signalling of DNA damage and how this promotes DNA repair. We have significant expertise in studying these processes in human cells and model organisms. The Research Technician will manage our eukaryotic cell culture library and other lab stocks in addition to providing experimental support to a variety of projects. Tasks will involve cell-based assays for DNA repair, using genome editing technologies to generate cell lines and recombinant DNA technology. The Research Technician will also act as laboratory manager for the research group, proactively maintaining stocks, equipment and facilities, health and safety protocols, and creating an effective working environment. This full-time fixed-term post is funded by the Medical Research Council up to 6th June 2025 in the first instance. The actual starting salary offered will be based on qualifications and relevant skills acquired and will also be determined by the funding available. **About you** You will hold a BSc Degree in an area of molecular and cell biology, genetics or biochemistry. You should have experience of mammalian cell culture, including clonal cell line generation and recombinant protein expression using mammalian expression systems. You will also have experience of employing cell- based assays to assess mechanisms of cell function. Experience in manipulating gene expression by siRNA and/or genome editing is also desirable. You will have experience of working in laboratory environment and be able to work effectively with other lab members as part of a team. Good organisational and management skills are expected in order to manage facilities and equipment effectively, together with being proactive and capable of taking the lead in maintaining an effective and orderly lab environment. **Application Process** You will be required to upload a supporting statement, setting out how you meet the selection criteria, curriculum vitae and the names and contact details of two referees as part of your online application. Only applications received before **12 noon** on **Friday 6th October 2023** can be considered. Interviews for shortlisted candidates will be held as soon as possible thereafter. **Committed to equality and valuing diversity** """ . . . . . . . . . . "address"@en . _:N66779a3ecf6d43a3a91840b7b7b31c6b . "Address"@en . .