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We are seeking a Postdoctoral Research Associate to work on a BBSRC grant. The research topic is ‘Elucidating the role of the CHLORAD machinery in chloroplast protein degradation’.

 

You will conduct ground-breaking research on the degradation of chloroplast proteins by the ubiquitin-proteasome system (UPS), focusing on how this is implemented to regulate the chloroplast protein import machinery. Our previous work identified a ubiquitin E3 ligase, termed SP1, in the chloroplast outer membrane that regulates chloroplast protein import by triggering the UPS-mediated degradation of import machinery components; and showed that SP1 cooperates with several other components in a proteolytic pathway termed CHLORAD (chloroplast-associated protein degradation). This project will elucidate the functions of the CHLORAD system in fine molecular detail.

 

Responsibilities include planning, troubleshooting, and expertly executing the research work involved in this project. You will present and discuss project data in the context of the relevant literature at lab meetings and contribute to the daily organisation and management of the research laboratory. You will analyse and prepare data and contribute to the preparation of manuscripts for publication, and research grant applications. Part of the role will also be to supervise research students and other junior researchers in the research group.

 

The successful applicant will hold, or be close to completion of, a PhD/DPhil and relevant experience. You will have experience of protein biochemistry and molecular biology, including membrane protein analysis. You will possess the following: high motivation with intellectual curiosity and rigour; ability to work independently and manage multiple tasks; ability to assimilate and evaluate the relevant literature and its implications; and willingness and ability to communicate with colleagues and to assist their work. You will have statistical and data analysis skills relevant to the project and the ability to present data to colleagues and non-specialist audiences. The ability to write effectively is essential.

 

Informal enquiries should be directed to Paul Jarvis at paul.jarvis@biology.ox.ac.uk

 

The University of Oxford is committed to equality and valuing diversity. All applicants will be judged on merit, according to the selection criteria.

 

This post is full time and available from 1 January 2025 until 1 May 2026.

 

The closing date for applications is 12.00 noon on Friday 22 November 2024, interviews are likely to be scheduled for late November 2024.

 

Applications for this vacancy are to be made online via our e-recruitment system, and you will be required to upload a supporting statement and CV as part of your online application.
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"""Job description Post Postdoctoral Research Associate Department Biology Division Mathematical, Physical and Life Sciences Division (MPLS) Location South Parks Rd, Oxford, OX1 3RB Grade and salary Researcher Grade 7: £38,674 - £46,913 per annum Hours Full time Contract type Fixed term (available from 1st January 2025 to 1st May 2026) Reporting to Paul Jarvis Application deadline Friday 15 November 2024 Vacancy reference 176394 Recruitment contacts HR: hr@biology.ox.ac.uk PI: Professor Paul Jarvis – paul.jarvis@biology.ox.ac.uk Additional information Whilst the role is a grade 7 position, we would be willing to consider candidates with potential but less experience who are seeking a development opportunity, for which an initial appointment would be at grade 6 (£34,982 - £40,855 per annum) with the responsibilities adjusted accordingly. This would be discussed with applicants at interview/appointment where appropriate. Research topic Elucidating the role of the CHLORAD machinery in chloroplast protein degradation Principal Investigator / supervisor Professor R. Paul Jarvis Project team Jarvis Group Project web site https://users.ox.ac.uk/~dops0547/ Funding partner The funds supporting this research project are provided by BBSRC Recent publications Li, N. and Jarvis, R.P. (2024) Recruitment of Cdc48 to chloroplasts by a UBX-domain protein in chloroplast-associated protein degradation. Nat. Plants 10: 1400-1417. Sun, Y. and Jarvis, R.P. (2023) Chloroplast proteostasis: import, sorting, ubiquitination, and proteolysis. Annu. Rev. Plant Biol. 74: 259-283. Ling, Q., Broad, W., Trösch, R., Töpel, M., Demiral Sert, T., Lymperopoulos, P., Baldwin, A. and Jarvis, R.P. (2019) Ubiquitindependent chloroplast-associated protein degradation in plants. Science 363: eaav4467. Ling, Q. and Jarvis, P. (2015) Regulation of chloroplast protein import by the ubiquitin E3 ligase SP1 is important for stress tolerance in plants. Curr. Biol. 25: 2527-2534. Jarvis, P. and López-Juez, E. (2013) Biogenesis and homeostasis of chloroplasts and other plastids. Nat. Rev. Mol. Cell Biol. 14: 787-802. Ling, Q., Huang, W., Baldwin, A. and Jarvis, P. (2012) Chloroplast biogenesis is regulated by direct action of the ubiquitin-proteasome system. Science 338: 655-659. Technical Skills Molecular biology; protein biochemistry; proteomics; plant genetics; plant physiology The role The human population is growing rapidly and set to exceed 9 billion by 2050, and there is ever increasing pressure on natural resources. Thus, the drivers for increased crop yields and resilience to climate change and sub-optimal growing conditions are stronger than ever. To meet these demands it will be essential to develop improved crop varieties through the application of novel technologies. Fundamental plant biology research in areas linked to plant yield and plant resilience will have a vital role to play in achieving these goals. Through research on the model plant Arabidopsis thaliana (thale cress), we have made some significant breakthroughs that are relevant in this regard. We discovered a gene called SP1 that controls important aspects of plant growth, including plant responses to adverse environmental conditions such as water stress and high salinity. Arabidopsis plants can be made more tolerant of environmental stresses by modifying SP1 expression. Our subsequent research identified several other genes that function in the same regulatory pathway as SP1, and we named this new pathway CHLORAD (for “chloroplast-associated protein degradation”). The CHLORAD system regulates the development of structures inside plant cells called chloroplasts. Chloroplasts are normal cellular constituents (organelles), and in many ways they define plants. They contain the green pigment chlorophyll and are responsible for photosynthesis, capturing sunlight energy and using it to power the activities of the cell. As photosynthesis is the only significant mechanism of energy-input into the living world, chloroplasts are of huge importance, not just to plants but to all life on Earth. Chloroplasts also have critical roles in plant responses to abiotic stress, and so are ideal targets for engineering stress tolerance in crops. Chloroplasts are composed of thousands of different proteins, and most of these are encoded by genes in the cell nucleus and so are synthesized outside of the organelle in the cellular matrix known as the cytosol. As chloroplasts are each surrounded by a double-membrane envelope, sophisticated machinery is needed to bring about the import of these proteins into the organelle. This comprises two molecular machines, one in each membrane, called TOC (for “Translocon at the Outer membrane of Chloroplasts”) and TIC (for “Translocon at the Inner membrane of Chloroplasts”). Each machine is composed of several different proteins that work cooperatively. The SP1 gene encodes a regulatory factor called a ubiquitin E3 ligase. Such regulators work by labelling-up unwanted proteins with a small tag called ubiquitin, to target them for removal. The SP1 E3 ligase mediates the removal of TOC components, and thereby controls TOC functions so that only the desired proteins are imported by chloroplasts. Such control enables major functional changes of chloroplasts during development and in adaptation to stress. But TOC proteins are deeply embedded in the chloroplast outer membrane, presenting a physical obstacle to their removal following labelling by SP1. We discovered a multicomponent “retrotranslocation” system that extracts proteins from the chloroplast membrane to overcome this obstacle. The SP2 gene encodes a channel across the chloroplast outer membrane, and our results show that it forms the exit gate for the removal of unwanted TOC proteins. Other key components of the retrotranslocation system are the cytosolic ATPase called Cdc48, and an outer membrane protein that acts to recruit Cdc48 to the chloroplast surface (a protein called PUX10). This project will study the CHLORAD machinery in detail to understand more clearly how it targets chloroplast proteins for degradation, elucidating molecular mechanisms and functions. Responsibilities • To plan, troubleshoot, and expertly execute the research work involved in this project. • To actively foster the intellectual environment of the research group through interaction with colleagues. • To present and discuss project data in the context of the relevant literature at lab meetings. • To contribute to the daily organisation and management of the research laboratory. • To analyse and prepare data and contribute to the preparation of manuscripts for publication, and research grant applications. • To supervise research students and other junior researchers in the research group. • To contribute to outreach and impact related activities relevant to the project. • Ad-hoc teaching and demonstrating • Embed the principles of mutual respect, equality, diversity, inclusivity and sustainability in all aspects of your work; undertake training as and when asked to do so. • Teach or undertake ad-hoc teaching (this may include lecturing, demonstrating, small group teaching, tutoring of undergraduates and graduate students and supervision of projects). Selection criteria Essential selection criteria • Hold, or be close to completion, of a relevant PhD/DPhil, in a relevant area • Experience of protein biochemistry, including membrane protein analysis. • Experience of molecular biology. • High motivation with intellectual curiosity and rigour. • Possession of a publication record in a relevant area. • Ability to work independently and manage multiple tasks. • Ability to assimilate and evaluate the relevant literature and its implications. • Willingness and ability to communicate with colleagues and to assist their work. • Track record of social responsibility in the laboratory. • Statistical and data analysis skills relevant to the project. • Ability to present data to colleagues and non-specialist audiences. Desirable selection criteria • • • Experience of proteomics or structural biology Experience of plant biology Experience of research on subcellular organelles, such as chloroplasts. 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"2024-11-22T12:00:00+00:00"^^ . "page" . . . """We are seeking a Postdoctoral Research Associate to work on a BBSRC grant. The research topic is ‘Elucidating the role of the CHLORAD machinery in chloroplast protein degradation’. You will conduct ground-breaking research on the degradation of chloroplast proteins by the ubiquitin-proteasome system (UPS), focusing on how this is implemented to regulate the chloroplast protein import machinery. Our previous work identified a ubiquitin E3 ligase, termed SP1, in the chloroplast outer membrane that regulates chloroplast protein import by triggering the UPS- mediated degradation of import machinery components; and showed that SP1 cooperates with several other components in a proteolytic pathway termed CHLORAD (chloroplast-associated protein degradation). This project will elucidate the functions of the CHLORAD system in fine molecular detail. Responsibilities include planning, troubleshooting, and expertly executing the research work involved in this project. You will present and discuss project data in the context of the relevant literature at lab meetings and contribute to the daily organisation and management of the research laboratory. You will analyse and prepare data and contribute to the preparation of manuscripts for publication, and research grant applications. Part of the role will also be to supervise research students and other junior researchers in the research group. The successful applicant will hold, or be close to completion of, a PhD/DPhil and relevant experience. You will have experience of protein biochemistry and molecular biology, including membrane protein analysis. You will possess the following: high motivation with intellectual curiosity and rigour; ability to work independently and manage multiple tasks; ability to assimilate and evaluate the relevant literature and its implications; and willingness and ability to communicate with colleagues and to assist their work. You will have statistical and data analysis skills relevant to the project and the ability to present data to colleagues and non-specialist audiences. The ability to write effectively is essential. Informal enquiries should be directed to Paul Jarvis at paul.jarvis@biology.ox.ac.uk The University of Oxford is committed to equality and valuing diversity. All applicants will be judged on merit, according to the selection criteria. This post is full time and available from 1 January 2025 until 1 May 2026. The closing date for applications is 12.00 noon on Friday 22 November 2024, interviews are likely to be scheduled for late November 2024. Applications for this vacancy are to be made online via our e-recruitment system, and you will be required to upload a supporting statement and CV as part of your online application. """ . .